Effects of ubiquitin-proteasome inhibitor MG-132 on proliferation and apoptosis of gastric carcinoma cells

AIM: To investigate effects of ubiquitin-proteasome inhibitor on proliferation and apoptosis of gastric carcinoma cells. METHODS: The gastric carcinoma cells SGC-7901 were treated with MG-132. The effects of growth suppression on cells were evaluated with MTT assay. Apoptosis was measured by flow cytometry. Expression of survivin was detected by immunocytochemical technique. RESULTS: MG-132 showed obvious inhibitory effect on the growth of gastric carcinoma cells, IC_(50) of 24 and 48 h were 80.9 and (9.1) μmol·L~(-1) respectively. After treated with (5.0 μmol)·L~(-1) MG-132 for 24, 48, 72 and 96 h, the ratios of apoptotic cells were (4.2±0.6) %, (30±4) %, ((47±6) %) and (53±6) % respectively. Expression of survivin was high in gastric carcinoma cells and it was decreased in cells treated with MG-132. CONCLUSION: MG-132 can significantly inhibit the proliferation of gastric carcinoma cells and induce apoptosis with down ward regulation of survivin expression.