Comparative studies on isozymes of three strains of Carassius auratus

The expressions patterns of five isozymes(LDH,MDH,ME,EST and SOD) were investigated in different tissues of Pengze crucian carp(Carassius auratus),silver crucian carp D(C.auratus gibelio),and wild crucian carp(C.auratus),respectively,using polyacrylamide gel electrophoresis.Two bands(LDH7’and LDH8’) of LDH isozyme in liver tissue of Pengze crucian carp were more than those of silver crucian carp D.The main differences in SOD existed at heart and kidney,which showed one more band(SOD12’) of Pengze crucian carp than those of silver crucian carp D.The results indicated that Pengze crucian carp is different from sliver crucian carp in biochemical level;they perhaps come from different region and have different ancestor.Meanwhile,the electrophoretogram of Pengze crucian carp and silver crucian carp all included the basal patterns of wild crucian carp.Specially,EST isozyme patterns between Pengze crucian carp and wild crucian carp were similar among the patterns of several isozyme.It is presumed that Pengze crucian carp and silver crucian carp were all originated from wild crucian carp.The relation between Pengze crucian carp and wild crucian carp is closer than that of silver crucian carp and wild crucian carp.