Comparative study of the characteristics of epileptiform discharges in the hippocampal slices and combined entorhinal cortex-hippocampal slices

Objective In order to explore the Mg2 +-free artificial cerebrospinal fluid(ACSF) induced different epileptiform discharge patterns in adult mouse hippocampal slices and combined entorhinal cortex- hippocampal slices in vitro.Methods Two brain slice models were prepared,and Mg2 +-free-ACSF was used to induce epileptiform discharges,which were recorded by micro-electrode array(MEA).The spatiotemporal characteristics of the discharge patterns were studied following successful induction of epileptiform discharges in the two slice models.Results Mg2 +-free-ACSF induced interictal discharges in the hippocampal slice,with frequency of(11.6 ±2.4) times/min,and lasted 149.0-202.6 ms.While in the combined entorhinal cortex-hippocampal slice,the discharge pattern was alternated between interictal and ictal discharges.The frequency of interictal discharges was(12.9 ±3.3) times/min,with duration of 181.3-223.7 ms.The frequency of ictal discharges was(0.26 ± 0.07) times / min,with duration of 14.3-14.5 s.Conclusion Interictal as well as ictal discharges could be recorded in the combined entorhinal cortex-hippocampal slice network level.So the combined entorhinal cortex-hippocampal slice is an ideal model for epilepsy research.