Study of effects on lung with ulinastatin in rat of ventilation-induced lung injury

【Objective】To explore the effects of ulinastatin(Uti) for lung in healthy rat with ventilation induced lung injury.【Method】Forty healthy SD rats were randomly divided into five groups,with eight animals in each:sham group(C); large tidal volume ventilation group(M); pretreatment of Uti with doses of 10 ku/kg(U1),50 ku/kg(U5),100 ku/kg(U10). Except C group,others received ventilation for 4 h(parameter setting:Vt=30 ml/kg,RR= 40 breaths/min,I/E=1∶3,Fio2=21%). After ventilated for 4 h,the histological changes of the lungs were observed under light microscope. The concentrations of proteins,the counts of the total leukocyte and the differential counts in BALF were determined. 【Result】①With 4 h ventilated,PaO2 in the M group were significantly reduced compared with baseline values. Compared with C group,the lung W/D ratio; the level of proteins; the total numbers of leukocytes and leukocyte differential counts in BALF were great elevated. ②Prior treatment with Uti lessened all above index in different degrees. There were no statistical differences between the U1 group and the M group,but the differences between the U5 and U10 group with M group were distinct.【Conclusion】Uti could attenuate the lung injury from high tidal volume ventilation through suppressing the recruitment and activation of neutrophil and reducing the proteinum effusion for pneumoangiogram. The effects of lung in rat are dose dependent.