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2010•Journal of Tropical OrganismsRequires access

Optimization on the Preparation of the Competent Cells of Escherichia coli HB101

Chao Wang

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Abstract

The strain source,the growth phrase of the cell and the storage condition were optimized for preparing the competent cells of Escherichia coli HB101. The results revealed that the highest transformation ability could be obtained when the source of the strain was streaked from strains stored under-70 ℃,cells were harvested when OD600 were 0.3—0.4,the storage condition of competent cells was under 4 ℃ for 1 d and-70 ℃ for 1 month,and the highest transformation efficiency attained 1.18×107 cfu·g-1 DNA.

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The strain source,the growth phrase of the cell and the storage condition were optimized for preparing the competent cells of Escherichia coli HB101. The results revealed that the highest transformation ability could be obtained when the source of the strain was streaked from strains stored under-70 ℃,cells were harvested when OD600 were 0.3—0.4,the storage condition of competent cells was under 4 ℃ for 1 d and-70 ℃ for 1 month,and the highest transformation efficiency attained 1.18×107 cfu·g-1 DNA.

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Available abstract

The strain source,the growth phrase of the cell and the storage condition were optimized for preparing the competent cells of Escherichia coli HB101. The results revealed that the highest transformation ability could be obtained when the source of the strain was streaked from strains stored under-70 ℃,cells were harvested when OD600 were 0.3—0.4,the storage condition of competent cells was under 4 ℃ for 1 d and-70 ℃ for 1 month,and the highest transformation efficiency attained 1.18×107 cfu·g-1 DNA.

Key concepts: Escherichia coli, Transformation (genetics), Strain (injury), Biology, Microbiology, Biotechnology, Food science, Biochemistry

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