Construction of eukaryotic expression plasmid of glycoprotein E gene of pseudorabies virus Min-A strain

With a pair of primers designed according to the published sequence of pseudorabies virus (PRV) Rice strain, the fragment encoding glycoprotein E(gE) of PRV Min A strain was amplified specifically by PCR. The fragment was inserted into cloning vector pUC19, then, the correct gE gene was inserted into pFastBac donor plasmid , recombinant pFastBac gE was obtained.The plasmid was transformed into shuttle plasmid Bacmid, the expression vector Bacmid gE was obtained.