Effect of Long-Term Intervention with Proanthocyanidin on Oxidative Stress in Type II Diabetes

Objective: To observe the oxidative stress in Type Ⅱ diabetic rats after long-term feeding of grape seed proanthocyanidin extract(GSPE) and the effect of GSPE intervention on oxidative stress.Methods: Forty-eight Type Ⅱ diabetic rats that were successfully induced were randomly divided into 4 groups: three GSPE-treated groups and one diabetic model group.Twelve rats fed on normal forage were served as normal control group.GSPE-treated groups were administered with GSPE by gavage at doses of 125,250,500 mg/(kg.d).Diabetic model group and normal control group were administered with deionized water also by gavage.After 24 weeks,all rats were killed and blood and organs were acquired.Oxidative indicators(SOD,GSH-Px and MDA) of all tissues were detected.Results: Compared with the normal control group,SOD activity in the serum and liver tissue of the diabetic model group was significantly decreased,so was the GSH-Px activity in liver and muscle tissue,but the amount of MDA in the brain of the diabetic model group was increased;compared with the diabetic model group,SOD activity in the serum,liver and spleen and GSH-Px activity in the serum,liver,muscle and spleen from the intervention groups was significantly increased(P 0.05).Conclusion: The level of oxidative damage in different tissues from Type Ⅱ diabetic rats is different.Long-term intervention with GSPE can reduce oxidative damage to tissues.