Effect of parthenolide on proliferation,apoptosis and migration of human hepatocellular carcinoma HepG-2 cells

Objective To observe the effect of parthenolide on the proliferation,apoptosis and migration of human hepatocellular carcinoma HepG-2 cells. Methods In the experimental group,2. 5,5,10,20 and 40 μg /mL of parthenolide were respectively added into HepG-2 cells,but in the control group,no parthenolide was added. The growth inhibitory rate of HepG-2 cells was calculated,the cell morphological changes were observed under a fluorescence microscope,the cell cycle and apoptosis were detected by flow cytometry before and after parthenolide,and cell scratch test was used to detect the effect of parthenolide on cell migration. Results HepG-2 cell proliferation was inhibited after being treated by parthenolide and in a time- and dose- dependence( all P 0. 05); the morphological changes were observed after 48-hourtreatment of 5 μg /mL parthenolide,the cytoplasm reduced,nuclear chromatin became thinner,and apoptotic bodies appeared. The cell percentage of the experimental group and the control group in G0/G1phase were 73. 36% ± 9. 13%,and 59. 28% ± 8. 37%,in S phase were 18. 34% ± 6. 09% and 27. 36% ± 4. 26%,in G2/M phase were 9. 36% ± 2. 98%and 14. 30% ± 3. 07%,and the apoptosis rates were 27. 45% ± 4. 15% and 0. 56% ± 0. 72%( all P 0. 05). The migration ability in the experimental group was lower than that in the control group( P 0. 05). Conclusion Parthenolide inhibits the proliferation and induces the apoptosis of HepG-2 cells by arresting the cells in G0/G1phase,and parthenolide has significant anti-migration effect on HepG-2 cells.