Evaluation of blood-brain barrier permeability after cerebral ischemia/reperfusion in rats

【Objective】 To explore change of blood brain barrier(BBB) and formation edema at early period after cerebral ischemia/reperfusion in rats. 【Methods】 Damage to the BBB was judged by extravasation of Evans Blue(EB) dye.Cerebral infarction volumes were quantitated by TTC staining and brain water content was determined by dry/wet weight method. 【Results】 At 3h of reperfusion after cerebral ischemia,the permeability of BBB began to increase(P0.05),reached the peak at 24h of reperfusion(P0.01).Comparing with normal side,the content of EB in the ischemia side were significant increased(P0.05).As compared to sham-operated group,the concentration of EB in ischemia-reperfusion groups was increased(P0.05).The change pattern of brain water content was similar to that of BBB permeability.However,cerebral infarction sizes in ischemia-reperfusion groups were not significant difference. 【Conclusion】 Integrality of the BBB is broken down at early period of cerebral ischemia/reperfusion,and exacerbated cerebral edema.