Inhibitory Effect of RNA Interference on Smad7 Gene Expression

The tiny RNAs are termed short interfering RNAs(siRNAs) depending on their origin. They down-regulate gene expression effectively by binding to complementary mRNAs and triggering mRNAs degradation(RNAi). The current research was designed to investigate whether siRNAs can inhibit the expression of Smad7 gene. Gene transfection and Northern blot were performed to determine the effect of gene inhibition of Smad7 by RNA interference in BEP2D and BERP35T2 cells. Meanwhile, fluoroscopy was used to evaluate impact of GFP siRNAs on GFP expression. It could be conclude that RNAi can inhibit specific expression of Smad7 gene and GFP gene. The results lay foundation for further study on function of Smad7 gene and TGF-β signal transduction pathway.