ScholarLens Back to search
2010•Chinese Archives of Otolaryngology-head and Neck SurgeryRequires access

Study on the effect of EGCG-induced apoptosis in squamous cell carcinoma of the head and neck

Xu Ting

Open publisher page 0 citations

Abstract

OBJECTIVE To investigate the effect of epigallocatechin-3-gallate(EGCG)on the induction of apoptosis and its possible molecular mechanism in head and neck cancer cells. METHODS Head and neck cancer cells Tu177 and Tu212 were treated with EGCG at different concentration for 72 hours. Flow cytometry analysis was employed for apoptosis, and Western blot was used to detect the expression of apoptosis-related proteins. Meanwhile, Tu177 and Tu212 were treated with EGCG, AKT activity was detected at different time points. RESULTS EGCG induced apoptosis of head and neck cancer cells in a dose dependent manner, the expression levels of cleaved-caspase 9, -caspase 3, and PARP were obviously up-regulated in EGCG treated cells. At the same time, the expression of p-AKT was down-regulated in a time-dependent manner by EGCG. CONCLUSION EGCG could induce apoptosis in head and neck cancer cells possibly through AKT and mitochondrial mediated pathways.

About this research paper

What this paper is about

OBJECTIVE To investigate the effect of epigallocatechin-3-gallate(EGCG)on the induction of apoptosis and its possible molecular mechanism in head and neck cancer cells. METHODS Head and neck cancer cells Tu177 and Tu212 were treated with EGCG at different concentration for 72 hours. Flow cytometry analysis was employed for apoptosis, and Western blot was used to detect the expression of apoptosis-related proteins. Meanwhile, Tu177 and Tu212 were treated with EGCG, AKT activity was detected at different time points. RESULTS EGCG induced apoptosis of head and neck cancer cells in a dose dependent manner, the expression levels of cleaved-caspase 9, -caspase 3, and PARP were obviously up-regulated in EGCG treated cells. At the same time, the expression of p-AKT was down-regulated in a time-dependent manner by EGCG. CONCLUSION EGCG could induce apoptosis in head and neck cancer cells possibly through AKT and mitochondrial mediated pathways.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

OBJECTIVE To investigate the effect of epigallocatechin-3-gallate(EGCG)on the induction of apoptosis and its possible molecular mechanism in head and neck cancer cells. METHODS Head and neck cancer cells Tu177 and Tu212 were treated with EGCG at different concentration for 72 hours. Flow cytometry analysis was employed for apoptosis, and Western blot was used to detect the expression of apoptosis-related proteins. Meanwhile, Tu177 and Tu212 were treated with EGCG, AKT activity was detected at different time points. RESULTS EGCG induced apoptosis of head and neck cancer cells in a dose dependent manner, the expression levels of cleaved-caspase 9, -caspase 3, and PARP were obviously up-regulated in EGCG treated cells. At the same time, the expression of p-AKT was down-regulated in a time-dependent manner by EGCG. CONCLUSION EGCG could induce apoptosis in head and neck cancer cells possibly through AKT and mitochondrial mediated pathways.

Key concepts: Apoptosis, Flow cytometry, Head and neck squamous-cell carcinoma, Western blot, Protein kinase B, Cancer research, Cancer cell, Poly ADP ribose polymerase

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Study on the effect of EGCG-induced apoptosis in squamous cell carcinoma of the head and neck — Research Paper | ScholarLens