Expression an sigifiance of VEGF-D in lung adenocarcinoma tissue

Objective To investigate the expression of vascular endothelial growth factor-D(VEGF-D) in lung adenocarcinoma.Methods The expression of VEGF-D mRNA was determined by the semi-quantitative reverse transcriptase-polymerase chain reaction(RT-PCR) method and the expression of VEGF-D,MLVD and MVD were determined by immunohistochemistry in 48 cases of lung adenocarcinoma.Results Expression of VEGF-D mRNA were higher in lung cancer than in normal lung tissues(P0.01),positive ratio of VEGF-D was 58.3%(28/48).The expression of VEGF-D in cancerous peripheral tissures were significantly higher than in the center of the tumors.There was no correlation between the expression of VEGF-D and differentiation.Stages Ⅲ～Ⅳ of lung adenocarcinoma showed strong expression of VEGF-D than that of stages Ⅰ～Ⅱ diseases.The MLVD and positive lymph node in positive VEGF-D group were higher than those in the negative group,but the MVD was same.Conclusions VEGF-D expression raises in lung adenocarcinoma.VEGF-D participate in the regulation of lymph angiogenesis,induce lymphangiogenesis and facilitate to lymphatic metastasis.