Impact of Tertramethylpyrazine on the Expression of Nuclear Factor-κB and the Activity of NOS after Situ Renal Ischemia-Reperfusion in Rats

Objective To observe the impact of tertramethylpyrazine(TMP) on the expression of Nuclear factor-κB and the activity of NOS after situ renal ischemia/reperfusion(I/R) in rats. Methods 30 female SD rats were randomly divided into sham operated group,model group and TMP treated group.Plasma samples were collected for Cr and BUN test.Renal tissues were collected to detect histological changes,the expression of NF-κB and the activity of NOS. Results Compared with the model group,the serum levels of Cr and BUN were decreased in the TMP group(15,30,45 mg·kg-1),among which 15 mg·kg-1 was more effective.Meanwhile,the expression of NF-κB and the activity of iNOS in renal tissues were decreased significantly. Conclusion TMP preconditioning can protect the renal function after renal I/R,and the mechanisms of which may be associated with inhibiting the activation of NF-κB and the expression of iNOS.The protective effect is dosage related,and the 15 mg·kg-1 seems to be the optimization.