Anti-tumor effects of AL3810 on A549 human lung adenocarcinoma

Objective To test the anti-tumor effect of AL3810 on A549 human lung adenocarcinoma.Methods ①The antiproliferative effects of AL3810 against 13 human carcinoma cell lines and MRC-5 cell line in vitro were observed by MTT assay.②The effects of AL3810 on cell cycle distribution and induction of apoptosis were assessed by flow cytometry analysis.③The effects of AL3810 on the active caspase-3 of A549 cell line were assessed by flow cytometry analysis.④The effect of AL3810 on cell migration was determined by wound assay.⑤The dose-effect relationship on antitumor effect of AL3810 was evaluated in vivo with the model of nude mice bearing A549 xenografts.Results AL3810 inhibited most of the cell lines significantly.AL3810 had the ability to induce apoptosis of A549 cell,and also showed in time dependent manner.In addition,AL3810 was also observed to block cells in G0-G1phase of cell cycle.AL3810 activated the caspase-3 in cells with a time dependent manner.Cell migration was also inhibited by AL3810 and the effects were time dependent.AL3810 showed significant inhibitive effects and the dose-effect relationship of tumor growth in xenografted nude mice.Conclusion AL3810 inhibited the proliferation of several tumor cell lines while differed in inhibition degree.The mechanisms of AL3810 might be associated with induction of tumor cells apoptosis,G0-G1 phase arrest,activation of caspase-3 and inhibiting the migration of A549 cell.