Studies on the Screening of Chitinase Producing Strain from Ocean and Its Optimization on Fermentation

A strain with high productivity of chitinase was screened through transparent circle method from sea mud of Dalian seaside and was identified as species Brevibacterium linens.After medium optimization and orthogonal experiments,which include carbon sources,nitrogen sources,temperature and pH,the chitinase activity was improved.The results showed that the optimized conditions for chitinase production were 1 g/L peptone,10 g/L Colloidal chitin,the NaCl of 20 g/L,culture at 28 ℃and pH 7.0.Under these conditions the chitinase activity can reach 0.508 U/mL,9 times higher than that of parent strain.