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2005•Journal of Jiangsu Clinical MedicineRequires access

DETECTING AND SEMI-QUANTITATIVELY DETERMINING THE CIRCULATING CANCER CELLS IN THE PERIPHERAL BLOOD OF LUNG CANCER PATIENTS

Yunqing Xie

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Abstract

Objective To evaluate the significance of detecting and semi-quantitatively determining the circulating cancer cells in the peripheral blood of lung cancer patients.Methods CK19mRNA expression was detected by nested RT-PCR in the peripheral blood from 60 lung cancer patients.Circulating cancer cells in the peripheral blood were semi-quantitatively determined.Results The positive detection was 48% for cancer patients of all stages and 12% for healthy adults as control.Only three control samples showed positive result.The relative number of circulating cancer cells of two patients decreased from 25 and 16 to 5 and 1 after one cycle of chemotherapy respectively.Conclusion Nested RT-PCR technique is a specific and sensitively method to detect micrometastasis in patients with lung cancer.The quantitative method sensitively estimated cancer cell numbers in the peripheral blood.

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What this paper is about

Objective To evaluate the significance of detecting and semi-quantitatively determining the circulating cancer cells in the peripheral blood of lung cancer patients.Methods CK19mRNA expression was detected by nested RT-PCR in the peripheral blood from 60 lung cancer patients.Circulating cancer cells in the peripheral blood were semi-quantitatively determined.Results The positive detection was 48% for cancer patients of all stages and 12% for healthy adults as control.Only three control samples showed positive result.The relative number of circulating cancer cells of two patients decreased from 25 and 16 to 5 and 1 after one cycle of chemotherapy respectively.Conclusion Nested RT-PCR technique is a specific and sensitively method to detect micrometastasis in patients with lung cancer.The quantitative method sensitively estimated cancer cell numbers in the peripheral blood.

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Available abstract

Objective To evaluate the significance of detecting and semi-quantitatively determining the circulating cancer cells in the peripheral blood of lung cancer patients.Methods CK19mRNA expression was detected by nested RT-PCR in the peripheral blood from 60 lung cancer patients.Circulating cancer cells in the peripheral blood were semi-quantitatively determined.Results The positive detection was 48% for cancer patients of all stages and 12% for healthy adults as control.Only three control samples showed positive result.The relative number of circulating cancer cells of two patients decreased from 25 and 16 to 5 and 1 after one cycle of chemotherapy respectively.Conclusion Nested RT-PCR technique is a specific and sensitively method to detect micrometastasis in patients with lung cancer.The quantitative method sensitively estimated cancer cell numbers in the peripheral blood.

Key concepts: Medicine, Micrometastasis, Lung cancer, Peripheral blood, Cancer, Peripheral, Circulating tumor cell, Cancer cell

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DETECTING AND SEMI-QUANTITATIVELY DETERMINING THE CIRCULATING CANCER CELLS IN THE PERIPHERAL BLOOD OF LUNG CANCER PATIENTS — Research Paper | ScholarLens