Research on rapid detection of methicillin resistant staphylococcus aureus by real-time PCR

Objective To establish a real-time polymerase chain reaction (real-time PCR) assay for rapid detection of methicillin resistant staphylococcus aureus(MRSA). Methods The special sequence of mecA gene of methicillin resistant staphylococcus was amplified and characterized with a pair primers and TaqMan probe. With detecting the nuc gene of staphylococcus aureus, the method of detecting MRSA was developed. Comparing with KB-Test, the method was evaluated by detecting 22 samples. Results The real-time PCR method was designed to sensitively detect and identify MRCoNS and MRSA. The total assay could be completed in 1.5 hours. 6 samples were positive detected by the real-time PCR method, two more than KB-Test. And the result for MRSA strain was coincident by the two methods. Conclusion RT-PCR detection methods provide a special, sensitive, rapid, reproducible and accurate method for quantitative detection of MRSA, and provide an important reference for clinical medication.