Effects of interleukin-17 on eosinophils in mice with asthma

Objective To investigate effects of interleukin-17(IL-17) and the IL-17 neutralization antibody on eosnophils and eotaxin-1 in asthma mice.Methods A total of 60 Babl/c female mice were randomly divided into five groups : the healthy group(group A),the control group(group B),the asthma group(group C),the IL-17 neutralization antibody group(group D) and the IL-17 group(group E),12 mice in each group.Mice in group B,C,D and E were sensitized and challenged with ovalbumin to establish asthma models.Pathological characteristics of lung tissues were observed using HE staining.Eotaxin-1 in mouse lung tissue homogenate was determined by ELISA.Group A was not treated with particular methods.Results Airway inflammation in asthmatic mice was mainly characterized by eosinophil and neutrophil infiltration,which was less observed in the normal control group.Concentrations of eotaxin-1 in lung tissue homogenate in group C,D,E,B and A were in the order from high to low.The difference between the five groups was significant(P0.05).Conclusion Exogenous IL-17 may inhibit infiltration of eosinophils in the lung of asthma mice.