Construction of eukaryotic expression vector pDisplay-hGITRaa_(1-165) and expression of hGITRaa_(1-165) protein in COS-7 cells

Objective: To construct extracellular region of human GITR eukaryotic expression vector pDisplay-hGITRaa1-165 and express hGITRaa1-165 protein in COS-7 cells.Methods: The hGITRaa1-165 gene fragment containing a signal peptide was cloned into eukaryotic expression vector pDisplay.The constructed recombinant plasmid pDisplay-hGITRaa1-165 was identified by restriction analysis and sequencing then transfected COS-7 cells in the mediation of liposome.The recombination protein was analyzed by Western blot. Results: A eukaryotic expression vector pDisplay-hGITRaa1-165 was successfully constructed.Western blot analysis proved the expression of hGITRaa1-165 in the supernatant of COS-7 cells transfected with the recombinant plasmid. Conclusion: The pDisplay-hGITRaa1-165 protein was successfully expressed in COS-7 cells,providing research foundation for biology of GITR.