Tea Callus Inducing Culture and Catechins Synthesis

Differentparts offresh tea leaves were induced in the differentdisinfection methods to produce callus. Meanwhile, The effects of different tea varieties, different medium and different hormone combinations on catechins synthesis were investigated. Results showed that the second tea leaves as explants, 35% sodium hypochlorite, soak for 18 minutes, could be obtained better efficiency of the induction. In the subculture, the catechin content of Camellia sinensis var. Jinmudan callus was the highest among tea callus. Except Camellia sinensis var. Huangmeigui, the catechin content in the other five tea varieties using B5 medium was higher than that using the MS medium. We obtained the maximum catechins production,when the B5 medium used for Camellia sinensis var. Jinmudan callus cultivation was supplemented with 2,4-D0.5 mg/Land KT1.0mg/L.