Effect of metformin on the proliferation of human osteoblasts and the mRNA expression of collagen I and LRP5

Objective To observe the effect of different concentrations of metformin on the proliferation of human osteoblasts and the mRNA expression of collagen Ⅰand LRP5,and to investigate the possible mechanism of the effect of metformin on human osteoblasts.Methods Human osteoblasts cultured in vitro were treated with different concentrations of metformin( 0,25,50,100,and 200μmol/L) for 72 hours. Then cell proliferation was assessed with CCK-8 colorimetric method. The mRNA expression of collagen Ⅰ and LRP5 was detected using fluorescent quantitative RT-PCR. Results Metformin could promote the proliferation of osteoblasts( P 0. 05),and also could promote the mRNA expression of collagen I and LRP5( P 0. 05). The promotion effect was highest at the concentration of 200μ mol / L,showing a dose-dependent manner. Conclusion In a certain range of concentration,metformin can promote the proliferation of human osteoblasts. It may regulate this progress by enhancing the mRNA expression of collagen Ⅰ and LRP5through the Wnt / LRP5 signal pathway.