ScholarLens Back to search
2013•Journal of clinical and experimental medicineRequires access

Study on the influence of IL-4 on the expression of inflammatory related factors in LPS-activated microglia cells in mice

Hua Wang

Open publisher page 0 citations

Abstract

Objective To explore the influence of IL-4 on the expression of TNF- α,IL- 1β,IL- 6 and MMP9 in LPS- activated murine microglia cells. Methods LPS- activated microglia cells were treated in vitro for 24 h in the presence of IL- 4. The levels of TNF- α, IL- 1β,IL- 6 and MMP9 in the supernatant of culture were measured by enzyme- linked immunosorbent assay( ELISA),and the expression on mRNA level of TNF- α,IL- 1β,IL- 6 and MMP9 had been detected by RT- PCR. Results The concentrations of TNF- α,IL- 1β,IL- 6 and MMP9 in the supernatant of culture and mRNA levels of TNF- α,IL- 1β,IL- 6 and MMP9 were evidently increased in LPS- activated microglia cells and they were prominently decreased by co- incubation with IL- 4. Conclusion According to this study in mice,IL- 4 shows neuroprotective function by inhibiting the expression of inflammatory related factors in activated microglia cells.

About this research paper

What this paper is about

Objective To explore the influence of IL-4 on the expression of TNF- α,IL- 1β,IL- 6 and MMP9 in LPS- activated murine microglia cells. Methods LPS- activated microglia cells were treated in vitro for 24 h in the presence of IL- 4. The levels of TNF- α, IL- 1β,IL- 6 and MMP9 in the supernatant of culture were measured by enzyme- linked immunosorbent assay( ELISA),and the expression on mRNA level of TNF- α,IL- 1β,IL- 6 and MMP9 had been detected by RT- PCR. Results The concentrations of TNF- α,IL- 1β,IL- 6 and MMP9 in the supernatant of culture and mRNA levels of TNF- α,IL- 1β,IL- 6 and MMP9 were evidently increased in LPS- activated microglia cells and they were prominently decreased by co- incubation with IL- 4. Conclusion According to this study in mice,IL- 4 shows neuroprotective function by inhibiting the expression of inflammatory related factors in activated microglia cells.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To explore the influence of IL-4 on the expression of TNF- α,IL- 1β,IL- 6 and MMP9 in LPS- activated murine microglia cells. Methods LPS- activated microglia cells were treated in vitro for 24 h in the presence of IL- 4. The levels of TNF- α, IL- 1β,IL- 6 and MMP9 in the supernatant of culture were measured by enzyme- linked immunosorbent assay( ELISA),and the expression on mRNA level of TNF- α,IL- 1β,IL- 6 and MMP9 had been detected by RT- PCR. Results The concentrations of TNF- α,IL- 1β,IL- 6 and MMP9 in the supernatant of culture and mRNA levels of TNF- α,IL- 1β,IL- 6 and MMP9 were evidently increased in LPS- activated microglia cells and they were prominently decreased by co- incubation with IL- 4. Conclusion According to this study in mice,IL- 4 shows neuroprotective function by inhibiting the expression of inflammatory related factors in activated microglia cells.

Key concepts: Microglia, MMP9, Medicine, In vitro, Tumor necrosis factor alpha, Incubation, Neuroprotection, Molecular biology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Study on the influence of IL-4 on the expression of inflammatory related factors in LPS-activated microglia cells in mice — Research Paper | ScholarLens