Expression of Fusion Protein of Interleukin-21 and Hepatitis B Virus Pre-S2 and S Antigen in 293T Cells

Objective: To construct eukaryotic expression plasmids carried mouse interleukin-21(IL-21) and hepatitis B virus pre-S2 and S(HBV S2S),and to investigate the expression of the plasmids in 293T cells.Methods: IL-21 and HBV S2S were amplified by polymerase chain reaction(PCR),and then they were cloned to pcDNA3 eukaryotic expression plasmid.Recombinant plasmid containing both IL-21 and HBV S2S were constructed by using molecular cloning.Plasmids were transfected into 293T cells by lipofectamine 2000.Expressions of IL-21 and HBsAg in supernatant and cells were tested by ELISA and Western Blot,respectively.Results: The sequences of insertion elements were confirmed by enzyme digestion and DNA sequencing.Three recombinant plasmids were named as pcDNA-IL-21,pcDNA-S2S,and pcDNA-IL-21-S2S,respectively.These plasmids could be expressed and secreted to related proteins in 293T cells.Conclusions: Recombinant eukaryotic expression plasmids containing IL-21 and HBV S2S were successfully constructed and could be expressed in eukaryotic cells.