Expression of exogenous interferon-γ gene in human hepatocellular carcinoma cells mediated by liposome

Objective To establish a recombinant eukaryotic expression vector containing human interferon-γ(IFN-γ) cDNA,and investigate the expression of IFN-γ gene in transfected hepatocellular carcinoma (HCC) cell lines. Methods pcDNA3-IFN-γ,established by subcloning IFN-γ cDNA into a mammalian expression vector pcDNA3,was mediated into HCC cell lines SMMC-7721 and QGY-7701 by lipofectamine. The transfected HCC cells were selected in RPMI1640 containing G418(400～700μg).RT-PCR analysis and ELISA assay were used for mRNA transcription and protein expression of IFN-γ gene. Results IFN-γ mRNA was detected only in pcDNA3-IFN-γ transfected cell lines, and high levels of IFN-γ protein were detected only in the supernatants of IFN-γ gene-modified cell lines. Conclusions pcDNA3-IFN-γ can stably expressIFN-γ cDNA in the transfected HCC cell strains