Effects of different extraction methods on volatile chemical constituents of the Elscholtzia kachinensis distributed in Guizhou Province

For analysizing and comparing the difference of the volatile chemical constituents of Elscholtzia kachinensis distributed in Guizhou Province,different extraction methods were used,including steam distillation and solid-phase microextrations methods,gas chromatography and mass spectrometry(GC/MS)together with spectral banks(NIST05 and WILEY275) to analysize and identify constituents,and area normalization method to determine the relative content of each constituent.Sixty-seven components were identified and 33 compounds were coexisted in the two kinds of extractions.45 compounds were found in the solid-phase microextrations,55 compounds were observed in the steam distillation extraction.There was not remarkable difference of volatile components checked in the E.kachinensis between the two extraction methods.The volatile components showed antimicrobial activities in vitro.The results provided the scientific evidences for exploitation of spiceberry E.kachinensis.