Effect of Nuclear Factor-κB Inhibitor Pyrrolidine Dithiocarbamate on Left Ventricular Remodeling After Acute Myocardial Infarction in Old Mice

Objective:To study the effect of nuclear factor-κB(NF-κB) inhibitor pyrrolidine dithiocarbamate(PDTC)on cardiac rupture and ventricular remodeling after acute myocardial infarction(AMI) with the underlying mechanisms in old mice. Methods:A total of 212 mice with the age of 18 months were used,AMI model was created by left coronary artery ligation and the mice were divided into two groups,n=106 in each group.AMI+PDTC group,the mice were intraperitoneally injected with PDTC 120 mg/(kg·d) and AMI group,the mice were intraperitoneally injected with the same volume of normal saline.The cardiac rupture rate within 7 days was examined by echocardiography,the mRNA expression of TNF-α was detected by RT-PCR,the protein expression of matrix metalloproteinase-9(MMP-9) was examined by zymography and the collagen content was measured by Sirius red staining at 3,7,and 14 days after AMI respectively. Results:The cardiac rupture rate in AMI group and in AMI+PDTC group were 35.3% vs.15.6%,P=0.000.Compared with AMI group,the left ventricular end diastolic diameter was decreased,the left ventricular weight was increased in AMI+PDTC group,P0.05 respectively.Compared with AMI group,in AMI+PDTC group,the protein expression of MMP-9 was decreased 3 and 7 days after AMI,P=0.000 respectively;the mRNA expression of TNF-α was decreased 7 days and 14 days after AMI,P=0.000 respectively.Compared with AMI group,the collagen content decreased in AMI+PDTC group 3 days and 7 days after AMI,P0.01 respectively. Conclusion:Nuclear factor NF-κB inhibitor PDTC may decrease the cardiac rupture rate and improve the ventricular remo-deling after AMI in old mice,which might be via inhibiting MMP-9 and TNF-α expression.