4'-demethyl-4-deoxypodophyllotoxin on Four Kinds of Tumor Cells Apoptosis and the Mechanism Research

Objective: Study of 4 '-demethyl-4-deoxypodophyllotoxin( No. 0derivatives) on Hela,MG-63,Hep G2,A549 four kinds of tumor cells for Bcl-2、Bax role; explore No. 0 derivatives on four tumor cells cycle and apoptosis. Methods Taking No. 0 derivatives as the observation group,0. 1% DMSO as the blank control group,paclitaxel as positive control group. Observed the changes after 48 h,respectively,the expression of Bcl-2 、Bax was detected by ELISA kit for the tumor cells; by using flow cytometry,PI single labeled effect method to detect the cell cycle and apoptosis. Results By ELISA kit testing showed that the observation group and positive control group compared with blank control group,Bcl-2 / Bax were obviously decreased,with a significant difference( P 0. 05); No. 0 derivatives action of four tumor cells after 48 h effect on cell cycle and apoptosis was detected by flow cytometry,results show that the percentage of G0 / G1 phase cells decreased,G2 / M cell percentage almost constant,while the percentage of S phase cells was increased. No. 0 derivatives arrest cells in S phase,and the sub-G1 cell apoptotic peak increased,further explains its induction of apoptosis. Conclusions No. 0 derivatives by up-regulation of Bax,down-regulation of Bcl-2 to promote the apoptosis of tumor cells; and arrest cells in S phase. Sub-G1 peak increased,show its effect of inducing cell apoptosis.