Effect of SOD Added in Dilution on Boar Semen Normal Temperature Preservation
Zhu Lin-we
Abstract
Zhu Lin-we
Abstract
SOD can scavenge oxygen free radicals and prevent cell aging. In order to select the best concen tration of SOD in Boar Semen's normal temperature preservation, four kinds of concentration(100, 200, 300, 400 IU/m L) of SOD were added in Modena and BTS. Diluting with high rate of boar semen, then stored at room temperature. Sperm motility were observed in four treatment groups every 24 h; The changes of H2O2, MDA, SOD content were detected in sperm when stored after 3 days. The results showed that the addition of 200 IU/m L SOD in Modena could significantly improve sperm motility of boar, and reduce the content of MDA and H2O2 in boar semen and enhance the SOD activity in semen(P 0.05), The sperm motility after stored 4 d was 0.657. While the addition of 300 IU/m L SOD in BTS could significantly improve the effect of boar semen ambient preservation, the sperm motility after stored 4 d was0.653. The right amount addition of SOD could improve the preservation of sperms, would it will produce toxic effects on sperms if the addition of SOD excess.
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SOD can scavenge oxygen free radicals and prevent cell aging. In order to select the best concen tration of SOD in Boar Semen's normal temperature preservation, four kinds of concentration(100, 200, 300, 400 IU/m L) of SOD were added in Modena and BTS. Diluting with high rate of boar semen, then stored at room temperature. Sperm motility were observed in four treatment groups every 24 h; The changes of H2O2, MDA, SOD content were detected in sperm when stored after 3 days. The results showed that the addition of 200 IU/m L SOD in Modena could significantly improve sperm motility of boar, and reduce the content of MDA and H2O2 in boar semen and enhance the SOD activity in semen(P 0.05), The sperm motility after stored 4 d was 0.657. While the addition of 300 IU/m L SOD in BTS could significantly improve the effect of boar semen ambient preservation, the sperm motility after stored 4 d was0.653. The right amount addition of SOD could improve the preservation of sperms, would it will produce toxic effects on sperms if the addition of SOD excess.
Key concepts: BOAR, Semen, Andrology, Sperm motility, Sperm, Motility, Chemistry, Superoxide dismutase