Effects of irbesartan on the renal expressions of CTGF and p27kip1 in streptozotocin-induced diabetic rats

Objective To observe the effects of irbesartan (Irb) on the renal expressions of CTGF and p27kip1 in STZ-induced diabetic rats.Methods SD rats were randomly divided into group C,group DN and group DNI.Each group was re-divided into 4 subgroupsand were sacrificedat 1, 2,4 and 8 weeks after the diabetes was induced.Blood glucose(BG),body weight(BW),24-hour urinary albumin excretion(24 h Ualb),creatinine clearance(Ccr),kidney weight(KW),index of kidney hypertrophy(KW/BW) were determined when the rats were sacrificedby the end of experiment.Glomerular tuft area(A_(G)),glomerular volume(V_(G)),proximal tubular area(A_(T)) at each time point,thickness of glomerular basement membrane(GBM) and tubular basement membrane(TBM) at the 8th week were measured by CMIAS(RY-2000).Renal expression of CTGF and p27kip1 were detected by immunohistochemistry staining(SP method).(Results) The levels of group DN and group DNI rats' BG were higher than group C,while their BW were lower than group C.The rats of group DN had much higher 24 hUalb,Ccr,KW,KW/BW,A_(G),V_(G),A_(T),GBM,TBM and the expressions of CTGF and p27kip1 than control rats,while Irb could inhibit the increase of those parameters.(Conclusion) At the early stage of diabetes Irb can inhibit the renal hypertrophy and the expressions of renal CTGF and p27kip1.