The Role of Tristetraprolin Dephosphorylation in the Inhibition LPS-induced Expression of Inflammatory Cytokines in Macrophage Foam Cells by ApoAI

Aim To observe the effect of apolipoprotein AⅠ( ApoAⅠ) on the expression of inflammatory cytokines and explore the role of tristetraprolin( TTP)-translational modification in the ApoAⅠinhibition macrophage-derived foam cells expression of inflammatory cytokines. Methods THP-1 macrophage-derived foam cells were treated with ApoAⅠand / or lipopolysaccharide( LPS). Enzyme linked immunosorbent assay was used to determine levels of interleukin-1β( IL-1β) in cell lysates. Real-time polymerase chain reaction was used to determine mRNA and degradation rate of IL-1β. Western blot was used to determine protein levels of TTP and p-TTP. Results ApoAⅠsignificantly inhibited the levels of LPSinduced macrophage-derived foam cells inflammatory cytokines,and influenced the expression of TTP and TTP dephosphorylation. Conclusion ApoAⅠinhibiting the expression of inflammatory cytokines in macrophage-derived foam cells may be involved in the dephosphorylation of TTP,which can bind and target ARE-containing mRNA for rapid degradation.