Effect of Supplementation of Different Estrous Cycle Goat Serum on in Vitro Development of Bovine Embryos

In order to increase the rate of bovine embryo blastocyst development in vitro, different estrous cycle serum of goats was used in culturing embryos. The serums were collected among the estrous cycle.D0,D2,D4 and D7(the day of estrous beginning is D0)goat serum, then sterilired and stored. The result shows the effect of the different serum on the cleavage rate of parthenogenetic embryo is not significant,but the blastocyst rate is 29.5%,32.4%,33.9% and 41.3%, respectively, D7 serum can significantly increase the blastocyst rate and expanded and hatched embryos come earlier.D0 serum can raise the cleavage rate of IVF embryo significantly, but cannot improve the blastocyst formation obviously. It shows that it has the discrepancy between the in vitro developmental competence of the parthenogenetic and IVF embryo,D7 serum was beneficial to later development of bovine embryos. After culturing 3 d without serum, the addition D7 serum can heighten the blastocyst rate of IVF embryo,percentage of blastocyst was 42.9%. This is a better culturing method for culturing embryo in vitro.