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2013•Journal of Jinan UniversityRequires access

Detection of SNPs and SSR of amylase(AMY) gene in Siniperca chuatsi

Tao Zhu

Open publisher page 1 citations

Abstract

The aim of our research is to detect the single nucleotide polymorphism(SNP) and simple sequence repeats(SSR) of amylase gene in Siniperca chuatsi.PCR-direct sequencing(PCR-DS) method was used to locate the SNPs and SSRs,then SNPs genotyping was carried out with created restriction sites PCR(CRS-PCR) and PCR-DS method,and polyacrylamide gel electrophoresis was used to check the polymorphism of SSR.The results indicated that there are one SNP locus A1 in intron 1 of AMY gene and three SNPs(A2,A3 and A4) in intron 5.In addition,a SSR locus B1 locating in intron 5 shows 4 alleles and 9 haplotypes in total.

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What this paper is about

The aim of our research is to detect the single nucleotide polymorphism(SNP) and simple sequence repeats(SSR) of amylase gene in Siniperca chuatsi.PCR-direct sequencing(PCR-DS) method was used to locate the SNPs and SSRs,then SNPs genotyping was carried out with created restriction sites PCR(CRS-PCR) and PCR-DS method,and polyacrylamide gel electrophoresis was used to check the polymorphism of SSR.The results indicated that there are one SNP locus A1 in intron 1 of AMY gene and three SNPs(A2,A3 and A4) in intron 5.In addition,a SSR locus B1 locating in intron 5 shows 4 alleles and 9 haplotypes in total.

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Available abstract

The aim of our research is to detect the single nucleotide polymorphism(SNP) and simple sequence repeats(SSR) of amylase gene in Siniperca chuatsi.PCR-direct sequencing(PCR-DS) method was used to locate the SNPs and SSRs,then SNPs genotyping was carried out with created restriction sites PCR(CRS-PCR) and PCR-DS method,and polyacrylamide gel electrophoresis was used to check the polymorphism of SSR.The results indicated that there are one SNP locus A1 in intron 1 of AMY gene and three SNPs(A2,A3 and A4) in intron 5.In addition,a SSR locus B1 locating in intron 5 shows 4 alleles and 9 haplotypes in total.

Key concepts: Single-nucleotide polymorphism, Genotyping, Genetics, SNP genotyping, Locus (genetics), Biology, Intron, SNP

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