Determination of Purine Derivatives and Creatinine in Yak's Urine and Plasma by HPLC

A method of high performance liquid chromatography(HPLC) coupled with UVD was developed for the determination of purine derivatives(PD),including allantoin,creatinine,uric acid,xanthine and hypoxanthine,and creatinine in white yak's urine and plasma samples.The separation and quantification of target compounds were achieved on Lichrospher RP-C18 Merck(4.6×250 mm,5 μm i.d.) and 5C18-AR-Ⅲ Waters(4.6×250 mm) columns,respectively,under the optimal conditions of using ammonium acetate as mobile phase with a flow rate of 1 mL/min at 25 ℃.The determination of the analytes was accomplished within 10 min.The limits of determination(LODs) for yaks' plasma and urine were in the range of 0.2-0.4 μmol/L,and the recoveries for yaks' plasma and urine were 81%-103% and 83%-104%,respectively.The method was rapid,accurate and effective,and could be applied in the simultaneous determination of PD and creatinine in yaks' plasma and urine sample.