Effects of nimodipine on the expression of AQP4 and MMP-9 in early cerebral hemorrhage rats

Objective:To discuss the expression of nimodipine on AQP4 and MMP-9 mRNA in early cerebral hemorrhage surrounding tissue and the relationship of secondary brain edema caused by cerebral hemorrhage and AQP4、MMP-9.Methods:Cerebral hemorrhage model of rats were divided into control group,cerebral hemorrhage group and nimodipine group.The water content of brain,AQP and MMP-9 mRNA were observed on 6h,1d,3d,5d,7d after intracerebral hemorrhage.Results:(1) On 3d in the ICH group,the brain water content peaked and on 7d it decreased but still higher than normal.On 3d in the nimodipine group,the brain water content peaked but still lower than ICH group.(2)AQP4 and MMP-9 of cerebral hemorrhage group was higher than that of control group at five points,whose expression gradually increased,peaked on 3d,obviously decreased on 7d.(3)Rat's Brain edema degree and expression of AQP4 and MMP-9 in nimodipine group were less than those in cerebral hemorrhage group,whose expression gradually increased,peaked on 3d.(4)The degree of intracerebral hemorrhage edema and AQP4 and MMP-9 mRNA were positively correlated.Conclusion:(1)MMP-9 and AQP4 involved in the process of hemorrhagic brain edema damage.(2)Nimodipine can inhibit the expression of AQP4 and MMP-9 so as to reduce the degree of brain edema.