Construction and identification of human phage single-chain antibody library for pneumoconiosis

Aim:To construct a human pneumoconiosis phage single-chain antibody(ScFv)library with diversity.Methods:Total RNA was extracted from the peripheral blood lymphocytes from the patients with pneumoconiosis,VH and VL genes were amplified by semi-nested PCR and were assembled to form ScFv by Linker and cloned into phagemid pCANTAB-5E,and then transformed into E.coli TG1 to construct a human peumoconiosis phage scFv antibody library.Results:VH gene families and VL gene families were successfully amplified.A large human antibody library containing 2.4× 107pfu/mL clones was created after rescuing the recombinant phagemids from the transformed E.coli TG1 cells.Conclusion:A human ScFv library is successfully constructed finally.