Effect of Isorhmnetin on Bcl-2 Gene Expression of HeLa Cell

Aim to investigate the inhibition effect of isorhmnetin on oncogene expression of bcl 2 and study to the mechanism of inhibition effect on cancer.Methods By techniques of cell culture in vitro, HeLa cells were treated by isorhmnetin in 20 μg/ml,then observed and analyzed by light microscopy, electronic microscopy, 3H TdR incorporate assay and Flow Cytometry (FCM). Results The growth of HeLa cells was markedly inhibited. The rate of 3H TdR incorporation and colony forming capacity both declined. Flow cytometry technique demonstrated that the rate of apoptosis of those cells increased. oncogene expression of bcl 2 markedly decreased, but bax oncogene protein markedly increased. Conclusions isorhmnetin could have effect of inhibiting growth onr ocervical cancer cells.