Resistance of Total Flavones of Epimedium leptorrhizum Stearn on rat Osteoporosis Induced by Retinoic Acid

Objective: To observe the effects of total flavones of Epimedium leptorrhizum Stearn( YYHC) on rat osteoporosis induced by retinoic acid. Method: Rats were divided into control group,model group,Xianling Gubao capsule group( 0. 3 g·kg-1) and YYH-C lower,middle,and high dose( raw materials 0. 7,1. 4,2. 8 g·kg-1) groups. Except the control group,each groups were administrated retinoic acid 70 mg·kg-1in the first 14 consecutive days. At the same time,the different treatment groups were given the corresponding drug to intervene for 28 days. The vertebrae bone and femur bone of left side were dissected to examine the parameters of bone,including bone mineral density( BMD),bone Ca,bone P,and ash weight / dry weight percentage; the femur bone of right side were dissected to assess the morphology of bone. Result: After 28 days,the BMD of vertebrae bone and ash weight / dry weight percentage of femur bone of the model rats were obviously lower,compared with control group( P 0. 01,P 0. 05). Ca and P content of bone of the model rats were also significantly lower than those of control group( P 0. 01). Morphological examination showed osteoporosis performance such as femoral cortical thinning,bone marrow cavity enlargement,the trabecular bone becaming thin,sparse and irregular,moreover,osteoblast number reduced. YYH-C 2. 8 g·kg-1group could significantly increase the BMD and ash weight / dry weight percentage of vertebrae bone( P 0. 01,P 0. 05),meanwhile,three treatment groups of YYH-C could increase the ash weight / dry weight percentage and Ca,P content of the bone,enlarge trabecular area and cortical bone area percentage( P 0. 01,P 0. 05). In addition,YYH-C 1. 4,2. 8g·kg-1groups could increase the thickness and area percentage of trabecular bone( P 0. 01),YYH-C of 1. 4 g·kg-1could reduce the number of osteoclasts( P 0. 05). Conclusion: The total flavones of E. leptorrhizum Stearn could resist osteoporosis on rat effectively by preventing the loss of bone mass on rat caused by retinoic acid,improving the microstructure of bone tissue,regulating the disorder of bone formation and bone destruction.