2009•Unpublished venueRequires access

The influence on VEGF expression by phosphatidylinositol 3-kinase signaling pathway in hepatocellular carcinoma cells

Jianmin Bian

Abstract

Objective:To investigate the roles of phosphatidylinositol 3-kinase(PI3K) in the regulation of expression of vascular endothelial growth factor(VEGF) in hepatocellular carcinoma(HCC) cells. Methods: The high metastasic cell line HCCLM3 were cultured in DMEM medium,HCCLM3 cells were divided into control groups and LY294002 groups(5μM,10μM,20μM),LY294002 was the specific inhibitor of PI3K.The expression of VEGF mRNA was detected by reverse transcriptional polymerase chain reaction(RT-PCR) after 6 hours. Results: In HCCLM3 cells,the expression of VEGF mRNA can be apparently inhibited by LY294002. With the increase of LY294002 concentration,the expression of VEGF mRNA decreased gradually(P0.01)and in a dose-dependent manner. Conclusion: LY294002 can reduce the expression of VEGF mRNA in HCCLM3 cell line,the expression of VEGF in HCC cells is regulated by PI3K.

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Key concepts: LY294002, Phosphatidylinositol, Hepatocellular carcinoma, Messenger RNA, PI3K/AKT/mTOR pathway, Kinase, Vascular endothelial growth factor, VEGF receptors