2012•Dalian Yike Daxue xuebaoRequires access
Expression of AQP-1 in acute lung injury induced by lipopolysacharide in rats
Xuemei Bai
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Abstract
[Objective]To investigate the expression and effect of AQP-1 in acute lung injury(ALI) induced by lipopolysaccharide.[Methods]Twenty Sprague-Dawley rats were randomly divided into LPS group treated with LPS injection and normal control group injected with saline(Control group).Arterial oxygen tension(PaO2) and wet/dry mass ratio(W/D) were tested at 6th hour after the injection.The expressions of AQP-1 protein in the lungs of rats were detected by immunohistochemistry and the expressions of AQP-1 mRNA were measured by a real-time fluorescence PCR.[Results]The arterial oxygen tension(PaO2) in LPS group was obviously lower than that in control group(55.07±17.02) mmHg vs(97.35±9.37) mmHg(P0.05).The wet/dry mass ratio(W/D) in LPS group was obviously higher than that in control group(4.93±0.16 vs 4.11±0.69,P0.05).The immunostaining showed a significant decrease in the expression of AQP-1 and a real time RT-PCR confirmed that the levels of AQP-1 mRNA were significantly reduced in LPS group(P0.01) at 6 h after LPS injection. [Conclusion]AQP-1 has an great effect in acute lung injury(ALI).