A Study on the relationship between HBV genotypes,YMDD mutation,and YMDD mutational patterns in patients with chronic hepatitis B(CHB) during lamivudine treatment

[Objective]This study aims to investigate the relationship between HBV genotypes,YMDD mutation,and YMDD mutational patterns in patients with chronic hepatitis B(CHB) during lamivudine treatment.[Method]One hundred and fifty-eight chronic hepatitis B patients treated with lamivudine for more than one year were employed in the study.HBV genotype was detected by the ELISA,HBV DNA was measured by fluorescence quantitative FCR,and YMDD mutations were analyzed by DNA sequencer.[Result]HBV genotype B and C accounted for 53.80%and 46.20%,respectively.Significant difference was found between patients of YMDD mutants with genotype B and C(43.84%vs 7.06%,P 0.01).The occurrence of YIDD mutant was lower in genotype B than that in genotype C((33.33%vs 81.25%,P 0.01),whereas YVDD mutant in genotype B(66.67%) was higher than that in genotype C(18.75%,P 0.01).[Conclusion]There was significant difference in development of YMDD mutation in different genotypes,and YMDD mutational patterns were different between genotypes B and C,which is valuable for lamivudine treatment against HBV in clinic.