Resveratrol Improving Insulin Sensitivity of Diabetic Rats by Increasing Protein Expression Levels of PPAR-γ and GluT-4 in Adipose Tissue

Objective To investigate the mechanism for resveratrol to improve the insulin sensitivity in diabetes mellitus.Methods Male Wistar rats were randomly divided into normal control group(NC,n=15) and diabetic group(DM,n=40) induced by STZ and high-fat diet.Diabetic rats were randomly divided into diabetic control group(DM-C,n=15) and diabetic group treated with resveratrol(DM-T,n=15).Then DM-T group was given intragastric administration with resveratrol at the dose of 50 mg/(kg·d)-1 for 11 weeks.FBG,fasting serum insulin(FIns),triglyceride(TG),total cholesterol(TC),insulin sensitivity index(ISI) and other biochemical parameters in each group were measured.The protein expressions of PPAR-γ and GluT-4 were determined by Western blot..Results At the end of the 17th week,compared with NC group,DM-C group showed higher FBG and TG,lower ISI,lower protein expressions of PPAR-γ and GluT-4 in the adipose tissue.Compared with DM-C group,DM-T group showed lower FBG,higher ISI.Integrated density(ID) showed higher protein expression of PPAR-γ of DM-T group in adipose tissue as compared with DM-C group.Integrated density(ID) showed higher protein expression of GluT-4 of DM-T group in adipose tissue as compared with DM-C group.Conclusion Resveratrol could increase the protein expressions of PPAR-γ and GluT-4 in adipose tissue,decreases FBG and improves insulin sensitivity of diabetic rats.