Molecular mechanism of invasion inhibitory effects of genistein on human ovarian serous papillary cystadenocarcinoma cell SKOV_3

Objective To investigate how genistein to inhibit the invasion of human ovarian serous papillary cystadenocarcinoma cell line SKOV3.Methods Millicell chamber and coculture method were used to establish chemotactic migration model in vitro to observe the effect of genistein on directional chemotactic migration movement of SKOV3 cells.The protein expressions of cell surface adhesion molecule CD44v6,matrix metalloproteinases and tissue inhibitor of metalloproteinases MMP-9/TIMP-1 and MMP-2/TIMP-2 were determined by using immunocytochemical method and their mRNA levels were examined by in situ hybridization.Results Compared with control group,the directional chemotactic migration of SKOV3 were significantly decreased after treated with 20 μmol/L and 40 μmol/L genistein,reached to(46.9±5.8)% and(28.3±4.7)% respectively(P0.01).genistein at 20 μmol/L down-regulated the protein and mRNA expressions of CD44v6,MMP-9 and MMP-2(P0.05),up-regulated those of TIMP-1 and TIMP-2(P0.05).Conclusion genistein exerts inhibitory effects on the invasion of SKOV3 cells by inhibiting chemotactic migration of the cells,that is,down-regulating the protein and mRNA expressions of CD44v6,MMP-9 and MMP-2,and up-regulating TIMP-1 and TIMP-2 expressions.