2002•Chinese journal of integrated traditional and Western medicineRequires access
Effect of Serum Containing Ruanganjian Decoction on Proliferation of HSC/T6 by Alarma Blue Assay
Dawei Tian
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Abstract
Objective:To evaluate the effect of serum containing Ruanganjian decoction on the proliferation of HSC/T6 by alarma blue assay.Methods:All of the sera were divided into 3 groups: calf serum group, rat serum group and drug-containing serum group. Each group was subdivided into three subgroups of 5 %, 10 % and 20 % (n=4 each). MTT assay and alarma blue assay were used to measure the effect of Ruanganjian decoction on the proliferation of HSC/T6.Results: In the groups assayed with MTT, rat serum of 5 % could inhibit HSC/T6 proliferation with more remarkable effect than calf serum (P 0.05), while rat sera of 10 % and 20 % with very significant difference (P 0.01); There was significant difference among different subgroups of drug-containing serum (P 0.01); As compared with rat serum, drug-containing sera of 5 % and 10 % could inhibit HSC/T6 proliferation with remarkable difference (P 0.05), while serum of 20 % with very significant difference (P 0.01). In the groups assayed with alamar blue, as compared with calf serum, rat and drug-containing sera of 10 % could inhibit HSC/T6 proliferation after 24 h with more significant difference (P 0.01); As compared with rat serum, drug-containing serum of 10 % could inhibit HSC/T6 proliferation with statistical difference (P 0.05); As compared with calf serum, rat serum of 10 % could inhibit HSC/T6 proliferation after 30 h with significant difference (P 0.05), while drug-containing serum of 10 % with more significant difference (P 0.01); As compared with rat serum, drug-containig serum of 10 % could inhibit HSC/T6 proliferation after 30 h with very significant difference (P 0.01). Conclusion: (1) Serum containing Ruanganjian decoction could inhibit the proliferation of HSC/T6, antagonize hepatic fibrosis. (2) Alarma blue assay was a creditable method. (3) Different animal sera had different effects on HSC proliferation and it was preferable to use a single animal serum in cultured medium.