Fermentation conditions and characteristics of antibiotic active substances by marine bacteria 201721

Objective Marine bacteria 201721 can produce strongly inhibitive active substances against E. coli. . Its fermentation conditions and chemical properties were studied. Methods Seven factors including glucose, yeast extract, peptone, medium pH, inoculum, agitation, and culture temperature were investigated by Plackett-Burman Design, and the significant factors including medium pH, peptone and culture temperature were optimized by using Response Surface Methodology. Results The predicted maximum inhibition zone (26. 5cm) were obtained under the optimized fermentation conditions (the medium pH, peptone and culture temperature were set at 6. 3 , 8. 2 g and 31. 6 C , respectively). The antibiotic active substances have better heat and pH stabilities and middle polarity via physicochemical experiments. Conclusion The proposed approach based on Plackett-Burman and Response Surface Methodology was suitable to optimize the fermentation condition of the strain.