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Effects of IL-6 on Apoptosis Induced by Hydrogen Peroxide in Alveolar Epithelial Cells

Binwu Ying

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Abstract

Objective:To observe the effects and mechanism of IL-6 on apoptosis induced by hydrogen peroxide in alveolar epithelial cells.Methods:A human alveolar epithelial A549 cells was used.MTT assay was used to determine the cell growth and survival rates.The number of apoptosis cells was detected by TUNEL.The expression levels of caspase-3 and caspase-9 proteins were detected by Western blot.Results:IL-6 significantly increased proliferation and decreased apoptosis of A549 cells.Compared with the model group,the expression levels of caspase-3 and caspase-9 proteins were decreased in IL-6-interfered group.Conclusion:IL-6 can inhibit apoptosis of A549 cells induced by hydrogen peroxide.It may be related to the low expression levels of caspase-3 and caspase-9 protein.

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What this paper is about

Objective:To observe the effects and mechanism of IL-6 on apoptosis induced by hydrogen peroxide in alveolar epithelial cells.Methods:A human alveolar epithelial A549 cells was used.MTT assay was used to determine the cell growth and survival rates.The number of apoptosis cells was detected by TUNEL.The expression levels of caspase-3 and caspase-9 proteins were detected by Western blot.Results:IL-6 significantly increased proliferation and decreased apoptosis of A549 cells.Compared with the model group,the expression levels of caspase-3 and caspase-9 proteins were decreased in IL-6-interfered group.Conclusion:IL-6 can inhibit apoptosis of A549 cells induced by hydrogen peroxide.It may be related to the low expression levels of caspase-3 and caspase-9 protein.

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Available abstract

Objective:To observe the effects and mechanism of IL-6 on apoptosis induced by hydrogen peroxide in alveolar epithelial cells.Methods:A human alveolar epithelial A549 cells was used.MTT assay was used to determine the cell growth and survival rates.The number of apoptosis cells was detected by TUNEL.The expression levels of caspase-3 and caspase-9 proteins were detected by Western blot.Results:IL-6 significantly increased proliferation and decreased apoptosis of A549 cells.Compared with the model group,the expression levels of caspase-3 and caspase-9 proteins were decreased in IL-6-interfered group.Conclusion:IL-6 can inhibit apoptosis of A549 cells induced by hydrogen peroxide.It may be related to the low expression levels of caspase-3 and caspase-9 protein.

Key concepts: Apoptosis, Hydrogen peroxide, A549 cell, TUNEL assay, Western blot, Medicine, Molecular biology, Caspase 3

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Effects of IL-6 on Apoptosis Induced by Hydrogen Peroxide in Alveolar Epithelial Cells — Research Paper | ScholarLens