Experimental study of Sanaoxiongting misture on the contents of PKC and MMP-9 in lung tissue of rat with asthma

ObjectiveTo investigate the effect of Sanaoxiongting misture on the contents of PKC and MMP-9 in lung tissue of rat with asthma.Methods 60 SD rats were randomly divided into six groups,control group,model group,high-,middle-,low-dose Sanaoxiongting misture group and Dexamethasone group,10 rats in each group.Besides control group,rat model of asthma were established in other groups.Equivalent volume 0.9% NaCl instead of mixture of OVA and aluminium hydroxide for sensitization was applied in control group.Ultrasonic nebulization inhalation of equivalent volume 0.9% NaCl instead of OVA for suscitation was applied in control group.30 min utes before suscitation corresponding medication was carried on in every treatment groups.Sanaoxiongting misture 20 g/kg,10 g/kg and 5 g/kg 4 mL were lavaged in high-,middle-,low-dose Sanaoxiongting misture group.Dexamethasone 1 mg/kg was intraperitoneal injected in Dexamethasone group.After two weeks,changes of pathomorphology in lung tissue and EOS and lymphocyte in bronchial mucosa and submucous were observed.PKC and MMP-9 in bronchus and lung tissue were detected.Results Bronchial submucous and smooth muscle layer in model group was thickened in comparison with that in control group.The alveolar diaphragm was widening,inflammatory cell infiltration were observed in airway wall and lung tissue.Improvement of inflammatory cell infiltration was observed in each treatment groups.The improvement of inflammatory cell infiltration in high-,middle-dose group and Dexamethasone group was superior to that in low-dose group(P0.05).EOS and lymphocyte in model group was higher than those in control group(P0.05).EOS and lymphocyte in high-,middle-,low-dose group and Dexamethasone group was lower than those in model group(P0.05).And EOS and lymphocyte in low-dose group was higher than those in Dexamethasone group(P0.05).PKC and MMP-9 in model group was higher than those in control group(P0.05).PKC and MMP-9 in high-,middle-,low-dose group and Dexamethasone group was lower than those in model group(P0.05).And PKC and MMP-9 in low-dose group was higher than those in Dexamethasone group(P0.05).Conclusion Sanaoxiongting misture can decrease obviously EOS and lymphocyte in tracheal mucosa and submucous,improve the pathological changes in lung tissue and reduce the expression of PKC and MMP-9.