Hydrolysis Process of Pupae Protein

The enzyme hydrolysis process of pupae protein is studied with bee pupae as raw material and the results are analyzed.The results show that the optimum conditions of the protein trypsin for hydrolysis of pupae protein are that: hydrolysis temperature is 60 ℃,enzyme concentration is 1.5%,reaction time is 1.5 h,pH value is 8.0,and ratio of material to water is 1∶8.In the optimum hydrolysis conditions,hydrolysis of the amino nitrogen content is 1.526 mg/mL.The optimum conditions of neutral protease for protein hydrolysis of pupae protein are that: hydrolysis temperature is 45 ℃,enzyme concentration is 2.0%,reaction time is 1 h,pH value is 7.5,and ratio of material to water is 1∶6.In the optimum hydrolysis conditions,hydrolysis of the amino nitrogen content is 2.068 mg/mL.The optimal enzyme is neutral protease enzymes.The optimum conditions of double enzymes for hydrolysis of pupae protein are that: the total amount of enzyme is 2.0%,enzyme ratio is 1∶2,hydrolysis temperature is 50 ℃,hydrolysis time is 2 h.In the optimal hydrolysis conditions,hydrolysis of the amino nitrogen content is 1.889 mg/mL.The influence of double enzymes hydrolysis is less than that of neutral protease.