Effects of Monosialoganglioside on the Expression of Nitric Oxide Synthase Positive Neurons in Hippocampus Dentate Gyrus Following Incomplete Cerebral Ischemia

Objective:To explore the regularity of the change of nitric oxide synthase(NOS) in hippocampus dentate gyrus by incomplete cerebral ischemia and reperfusion in different period,and investigate the effects of Monosialoganglioside on the change of the number of NOS-positive cells and the protection of neurons.Methods:The rat model of incomplete cerebral ischemia and reperfusion in different period were established by bilateral carotid artery clamping combined with blooding. NDAPH-diaphorase histochemistry was used to investigate the postischemia changes of NOS in dentate gyrus in the model and the effects of GM 1 on them were observed.Results:The number of NOS-positive cells in dentate gyrus was maximal(43.67±6.71) at 30min following incomplete ischemia and was decreased gradually at 2h、12h、24h、3d after reperfusion and was at normal level in 5d(30.92±5.43). GM 1 could inhibit their increases in different period of the ischemia and reperfusion in dentate gyrus.Conclusion:GM 1 could inhibit the expression of NOS-positive neurons in dentate gyrus following incomplete ischemia and reperfusion in different period.