Determination of p-coumaric acid in rat plasma by dispersive liquid-liquid microextraction-HPLC

Objective To establish an novel method for determination of p-coumaric acid in rat plasma by dispersive liquid-liquid microextraction coupled with high performance liquid chromatography(DLLME-HPLC).Methods The optimal conditions of dispersive liquid-liquid microextraction were as follow:ionic liquids of 1-hexyl-3-methylimidazolium hexafluorophosphate20 μL as extraction solvent,acetonitrile 80 μL as dispersive solvent,0.5 mol · mL-1H2SO4 solution as acidifying agent;extraction time was 1 min.Results Under the optimized conditions,the linear range of p-coumaric acid was 0.012-2.4 μg · mL-1(r=0.997 5),the limit of detection was 0.03 ng,relative recoveries were 96.7%-101.6% with both intra-day and inter-day RSDs less than 9%,extraction recoveries were 62.9-70.4%.Conclusions The method is sensitive and fast for determination of p-coumaric acid in rat plasma,and can provide a high degree of enrichment.