Studies on the cultivation method of sclerotium forming from hyphae of Grifola umbellata

OBJECTIVE To reveal the influence of different cultivation methods on sclerotium forming from hyphae of Grifola umbellata cultivated with companion fungus.METHODS The pure companion fungus,strain of G.umbellata and Armillaria mellea on tree trucks were inoculated in flowerpots and covered by sandy soil.RESULTS It was beneficial for sclerotium forming from hyphae of G.umbellata when inoculated its strain with companion fungus by interval method in cataphracted cuts of the tree trunk which late penetrated by rhizomorph of A.mellea. CONCLUSION Companion fungus was the key factor for sclerotium forming from hyphae of Grifola umbellata, and A.mellea was the main nutrition resource for continuing growth of sclerotia of G.umbellata.